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eclipse te-2000u epifluorescence inverted microscope  (Nikon)


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    Nikon eclipse te-2000u epifluorescence inverted microscope
    TIRFM configuration to study HCs transport to appositional and non-appositional plasma membrane zones. A representative cartoon showing two cells in juxtaposition and all the components involved in the data acquisition. TIRF microscopy configuration utilizes a critical angle, which creates a 100–200 nm-deep evanescent wave between the glass coverslip surface and cells to excite fluorophores located in the vicinity of the plasma membrane. Analysis was performed in the appositional and non-appositional zones ( a ). An example of how TIFR configuration is achieved under the microscopy is shown ( b ). Tagged connexins were illuminated in <t>epifluorescence</t> (left panel). Then, the surface reflective interference contrast (SRIC) filter ensures an appropriate field with cells attached to the glass (middle panel). Finally, it is switched to TIRF microscopy configuration for the data acquisition (right panel) ( b ).
    Eclipse Te 2000u Epifluorescence Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+te-2000u+epifluorescence+inverted+microscope/pmc11242593-186-8-5?v=Nikon
    Average 90 stars, based on 1 article reviews
    eclipse te-2000u epifluorescence inverted microscope - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Differential Regulation of Hemichannels and Gap Junction Channels by RhoA GTPase and Actin Cytoskeleton: A Comparative Analysis of Cx43 and Cx26"

    Article Title: Differential Regulation of Hemichannels and Gap Junction Channels by RhoA GTPase and Actin Cytoskeleton: A Comparative Analysis of Cx43 and Cx26

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25137246

    TIRFM configuration to study HCs transport to appositional and non-appositional plasma membrane zones. A representative cartoon showing two cells in juxtaposition and all the components involved in the data acquisition. TIRF microscopy configuration utilizes a critical angle, which creates a 100–200 nm-deep evanescent wave between the glass coverslip surface and cells to excite fluorophores located in the vicinity of the plasma membrane. Analysis was performed in the appositional and non-appositional zones ( a ). An example of how TIFR configuration is achieved under the microscopy is shown ( b ). Tagged connexins were illuminated in epifluorescence (left panel). Then, the surface reflective interference contrast (SRIC) filter ensures an appropriate field with cells attached to the glass (middle panel). Finally, it is switched to TIRF microscopy configuration for the data acquisition (right panel) ( b ).
    Figure Legend Snippet: TIRFM configuration to study HCs transport to appositional and non-appositional plasma membrane zones. A representative cartoon showing two cells in juxtaposition and all the components involved in the data acquisition. TIRF microscopy configuration utilizes a critical angle, which creates a 100–200 nm-deep evanescent wave between the glass coverslip surface and cells to excite fluorophores located in the vicinity of the plasma membrane. Analysis was performed in the appositional and non-appositional zones ( a ). An example of how TIFR configuration is achieved under the microscopy is shown ( b ). Tagged connexins were illuminated in epifluorescence (left panel). Then, the surface reflective interference contrast (SRIC) filter ensures an appropriate field with cells attached to the glass (middle panel). Finally, it is switched to TIRF microscopy configuration for the data acquisition (right panel) ( b ).

    Techniques Used: Clinical Proteomics, Membrane, Microscopy



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    eclipse te-2000u epifluorescence inverted microscope  (Nikon)
    90
    Nikon eclipse te-2000u epifluorescence inverted microscope
    TIRFM configuration to study HCs transport to appositional and non-appositional plasma membrane zones. A representative cartoon showing two cells in juxtaposition and all the components involved in the data acquisition. TIRF microscopy configuration utilizes a critical angle, which creates a 100–200 nm-deep evanescent wave between the glass coverslip surface and cells to excite fluorophores located in the vicinity of the plasma membrane. Analysis was performed in the appositional and non-appositional zones ( a ). An example of how TIFR configuration is achieved under the microscopy is shown ( b ). Tagged connexins were illuminated in <t>epifluorescence</t> (left panel). Then, the surface reflective interference contrast (SRIC) filter ensures an appropriate field with cells attached to the glass (middle panel). Finally, it is switched to TIRF microscopy configuration for the data acquisition (right panel) ( b ).
    Eclipse Te 2000u Epifluorescence Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+te-2000u+epifluorescence+inverted+microscope/pmc11242593-186-8-5?v=Nikon
    Average 90 stars, based on 1 article reviews
    eclipse te-2000u epifluorescence inverted microscope - by Bioz Stars, 2026-07
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    epifluorescence microscope eclipse te-2000u inverted microscope  (Nikon)
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    TIRFM configuration to study HCs transport to appositional and non-appositional plasma membrane zones. A representative cartoon showing two cells in juxtaposition and all the components involved in the data acquisition. TIRF microscopy configuration utilizes a critical angle, which creates a 100–200 nm-deep evanescent wave between the glass coverslip surface and cells to excite fluorophores located in the vicinity of the plasma membrane. Analysis was performed in the appositional and non-appositional zones ( a ). An example of how TIFR configuration is achieved under the microscopy is shown ( b ). Tagged connexins were illuminated in <t>epifluorescence</t> (left panel). Then, the surface reflective interference contrast (SRIC) filter ensures an appropriate field with cells attached to the glass (middle panel). Finally, it is switched to TIRF microscopy configuration for the data acquisition (right panel) ( b ).
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    eclipse te 2000u inverted epifluorescence microscope  (Nikon)
    90
    Nikon eclipse te 2000u inverted epifluorescence microscope
    TIRFM configuration to study HCs transport to appositional and non-appositional plasma membrane zones. A representative cartoon showing two cells in juxtaposition and all the components involved in the data acquisition. TIRF microscopy configuration utilizes a critical angle, which creates a 100–200 nm-deep evanescent wave between the glass coverslip surface and cells to excite fluorophores located in the vicinity of the plasma membrane. Analysis was performed in the appositional and non-appositional zones ( a ). An example of how TIFR configuration is achieved under the microscopy is shown ( b ). Tagged connexins were illuminated in <t>epifluorescence</t> (left panel). Then, the surface reflective interference contrast (SRIC) filter ensures an appropriate field with cells attached to the glass (middle panel). Finally, it is switched to TIRF microscopy configuration for the data acquisition (right panel) ( b ).
    Eclipse Te 2000u Inverted Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+te-2000u+epifluorescence+inverted+microscope/pm18339768-82-12-14?v=Nikon
    Average 90 stars, based on 1 article reviews
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    90/100 stars
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    Image Search Results


    TIRFM configuration to study HCs transport to appositional and non-appositional plasma membrane zones. A representative cartoon showing two cells in juxtaposition and all the components involved in the data acquisition. TIRF microscopy configuration utilizes a critical angle, which creates a 100–200 nm-deep evanescent wave between the glass coverslip surface and cells to excite fluorophores located in the vicinity of the plasma membrane. Analysis was performed in the appositional and non-appositional zones ( a ). An example of how TIFR configuration is achieved under the microscopy is shown ( b ). Tagged connexins were illuminated in epifluorescence (left panel). Then, the surface reflective interference contrast (SRIC) filter ensures an appropriate field with cells attached to the glass (middle panel). Finally, it is switched to TIRF microscopy configuration for the data acquisition (right panel) ( b ).

    Journal: International Journal of Molecular Sciences

    Article Title: Differential Regulation of Hemichannels and Gap Junction Channels by RhoA GTPase and Actin Cytoskeleton: A Comparative Analysis of Cx43 and Cx26

    doi: 10.3390/ijms25137246

    Figure Lengend Snippet: TIRFM configuration to study HCs transport to appositional and non-appositional plasma membrane zones. A representative cartoon showing two cells in juxtaposition and all the components involved in the data acquisition. TIRF microscopy configuration utilizes a critical angle, which creates a 100–200 nm-deep evanescent wave between the glass coverslip surface and cells to excite fluorophores located in the vicinity of the plasma membrane. Analysis was performed in the appositional and non-appositional zones ( a ). An example of how TIFR configuration is achieved under the microscopy is shown ( b ). Tagged connexins were illuminated in epifluorescence (left panel). Then, the surface reflective interference contrast (SRIC) filter ensures an appropriate field with cells attached to the glass (middle panel). Finally, it is switched to TIRF microscopy configuration for the data acquisition (right panel) ( b ).

    Article Snippet: Images were acquired using a Nikon Eclipse TE-2000U epifluorescence inverted microscope.

    Techniques: Clinical Proteomics, Membrane, Microscopy